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DNA polymerase requires a primer because it can't initiate polymerization by it self only,but requires a preexisting free 3'OH group to which it can add deoxynucleotides forming phosphodiester bond & the free 3'OH group is provided by the primer.Therefore the DNA polymerase requires primer.
Wiki User
∙ 13y ago
Wiki User
∙ 8y agoPrimers are the complimentary (single stranded) DNA molecule, these are designed to amplify the desired DNA fragment in the solution of whole DNA, that is present in PCR.
By selecting the specific primer, we can amplify the specific gene of interest through the whole genomic DNA strand.
Wiki User
∙ 14y agoPrimers are a strand of nucleic acid that serves as a starting point for DNA replication
Wiki User
∙ 13y agoBasically it is a protective coating to use before you paint.
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What is a primer in biology?
Primer is a piece of mRNA that replicates first in the DNA replication. The creation of primer is facilitated by the enzyme called PRIMASE which facilitates DNA replication along with HELICASE, an enzyme that breaks Hydrogen bonds to help unwind the DNA.
How does DNA replication differ in prokaryotes and eukaryotes?
1. In eukaryotic cells replication forks make several start sites along the DNA strand which forms replication "bubbles" which get larger the more DNA is copied, and stop when DNA replication is complete. In prokaryotic cell's DNA is formed in a loop, two replication forks start along one part of the loop (origin replication) and the replication forks copy DNA in opposite directions until they meet at the other side of the loop, making an exact copy of DNA.
What binds to the prokaryotic chromosome to start DNA replication?
Regulatory proteins bind to the prokaryotic chromosomes to start DNA replication.
What is the sequence of 3 RNA bases called?
Among many things an RNA primer for DNA replication.
DNA is copied during a process called?
Replication.
Produces The RNA primer required for DNA replication to begin?
the chemical primase produces the rna primer to start DNA replication. the primase is later removed and replaced with DNA by a repair polymerase
What does the synthesis of a new strand of DNA begin?
Following the initiation of DNA replication, the first step is the synthesis of a short RNA primer.
What are two differences between transcription and DNA replication?
# Transcription is copying a section of DNA (a gene) onto a mRNA molecule. Replication is the copying the entire DNA molecule. # Transcription does not require primer for initiation.DNA replication use primer for initiation. # RNA transcription, mRNA copy of the active stand of DNA helix is made this process is carried by different enzyme & result is a single of mRNA.DNA replication double helix of DNA is duplicated into two identical double helix which are also identical to the mother DNA, this process is carried by specific enzyme
What is a primer in biology?
Primer is a piece of mRNA that replicates first in the DNA replication. The creation of primer is facilitated by the enzyme called PRIMASE which facilitates DNA replication along with HELICASE, an enzyme that breaks Hydrogen bonds to help unwind the DNA.
What is the function of primer in PCR?
it synthesizes a single RNA primer at the 5' end of the leading end.
Why only RNA primer provide 3-OH' end for DNA replication?
The reason you only need RNA primer at the end to make a DNA replication is because it has a free 3' OH. RNA is ribonucleic acid. RNA contains long chains of nucleotides.
What binds to the prokaryotic chromosome to start DNA replication?
Regulatory proteins bind to the prokaryotic chromosomes to start DNA replication.
How does DNA replication differ in prokaryotes and eukaryotes?
1. In eukaryotic cells replication forks make several start sites along the DNA strand which forms replication "bubbles" which get larger the more DNA is copied, and stop when DNA replication is complete. In prokaryotic cell's DNA is formed in a loop, two replication forks start along one part of the loop (origin replication) and the replication forks copy DNA in opposite directions until they meet at the other side of the loop, making an exact copy of DNA.
Which of the following is needed in the process of DNA replication?
The ingredients needed for DNA replication include a short oligonucleotide primer and dNTPS. It also needs DNA polymerase and different transcription and translation factors.
What is the sequence of 3 RNA bases called?
Among many things an RNA primer for DNA replication.
DNA is copied during a process called?
Replication.
Why are RNA primers needed?
RNA primers are used to initiate the DNA replication at the template strand. DNA molecules require a free 3' OH, to which it could add the nucleotides. This free 3' OH is provided by the RNA primer. So prior to the synthesis of DNA a short fragment of RNA is synthesized that is later excised and filled with DNA molecules.
