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Use of trypan blue in cell counting?

Updated: 11/1/2022
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Jananisoundarya

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16y ago

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u count dead cells stained by trypan blue. trypan blue enters the cells only when it has died since when a cell dies it looses its property of controlling selective inlet of micromolecules. When cells r alive it doesn't allow inlet of trypan blue so viable cells r not stained. viable cells = total cells - trypan blues stained cells Nitin Ghoshal Dept of Biotechnology NEHU, Shillong, Meghalaya, India

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Q: Use of trypan blue in cell counting?
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