Is DNA accurate on siblings with the same mother but different fathers?

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I think what you mean to ask is "is DNA testing reliable to determine siblingship in two individuals with the same mother but not the same father?" The answer is, yes, but it depends. DNA is the material that codes for the genes which are essentially the blue print for how we are made. We have 46 chromosome, 23 we get from our mother and 23 from our father. We also get some mitochondrial DNA from our mother only. So testing depends on the individual sex of the children involved, but using mitochondrial DNA, it is quite accurate, since all children born of a certain female should share this DNA.
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