answersLogoWhite

0


Best Answer

a diagnostic test for the presence of the active ser is it's reaction with diisopropylphosphofluoridate(DIPF) which irreversibly inactivates the enzyme.Other ser residues including those on the same protein, do not interact with DIPF.

User Avatar

Wiki User

15y ago
This answer is:
User Avatar

Add your answer:

Earn +20 pts
Q: How will you establish that a given protein having hydrolase activity involves active serine residue at its active site?
Write your answer...
Submit
Still have questions?
magnify glass
imp
Related questions

What are examples of protein catalyst?

1) Protein disulfide isomerase or PDI:Catalyze the formation, isomerization and breakage of disulfide bonds in proteins 2) Peptidyl transferase: An aminoacyltransferase 3) palmitoyl[protein] hydrolase: palmitoyl[protein] + H2O palmitate + protein 4) Isocitrate lyase: converts isocitrate to succinate 5) Peptide synthases: biotin protein ligase (BPL), protein N-octanoyltransferases


Does total activity increase or decrease during protein purification?

Total Activity almost always decreases during protein purification.


Why is protein concentration necessary for accurate comparison of the enzyme activity of the fractions?

Enzyme activity sometimes reflects the amount of protein expressed in a cell--however, due to enzyme inhibitors, the enzyme activity is not always reflective of the amount of protein expressed by a cell.


Job of the ribosomes?

It involves in protein synthesis.It provide the surface for it.


How do you calculate specific activity of salivary amylase?

Divide the rate of enzyme activity by the protein concentration.


What is RAM protein?

A RAM protein is a protein that only forms a signaling network. Its function is to regulate Ace activity and cellular morphogenesis.


Which end C terminus and N terminus is important to the protein Activity?

To set a target protein in the cell


What does rhibosomes do in a plant cells?

It involves in protein synthesis.It is the general purpose.


what bacillus protein responsible for pro biotic activity?

acidophiles


Helps maintain the structure of chromosomes and control the activity of genes?

protein


How much protein does a pound of turkey have?

Enough Protein to give your body energy To do almost any physical activity


What is fold purification in protein purification?

A measure of how much more pure your protein is after a purification step in comparison to the crude. You can calculate this by dividing the purified steps specific activity by the crude steps specific activity.