a diagnostic test for the presence of the active ser is it's reaction with diisopropylphosphofluoridate(DIPF) which irreversibly inactivates the enzyme.Other ser residues including those on the same protein, do not interact with DIPF.
1) Protein disulfide isomerase or PDI:Catalyze the formation, isomerization and breakage of disulfide bonds in proteins 2) Peptidyl transferase: An aminoacyltransferase 3) palmitoyl[protein] hydrolase: palmitoyl[protein] + H2O palmitate + protein 4) Isocitrate lyase: converts isocitrate to succinate 5) Peptide synthases: biotin protein ligase (BPL), protein N-octanoyltransferases
Total Activity almost always decreases during protein purification.
Enzyme activity sometimes reflects the amount of protein expressed in a cell--however, due to enzyme inhibitors, the enzyme activity is not always reflective of the amount of protein expressed by a cell.
It involves in protein synthesis.It provide the surface for it.
Divide the rate of enzyme activity by the protein concentration.
A RAM protein is a protein that only forms a signaling network. Its function is to regulate Ace activity and cellular morphogenesis.
To set a target protein in the cell
It involves in protein synthesis.It is the general purpose.
acidophiles
protein
Enough Protein to give your body energy To do almost any physical activity
A measure of how much more pure your protein is after a purification step in comparison to the crude. You can calculate this by dividing the purified steps specific activity by the crude steps specific activity.