How do you denature the enzyme catalase?

Answer 1

AnswerFor any chemical reaction, the reaction rate increases with temperature, so the higher the temperature, the faster the rate. This is even true for exothermic equilibrium (reversible) reactions such as the Haber process: the position of the equilibrium may shift to favour the reverse reaction but the rate itself still increases. For any enzymatic reaction the reaction rate will increase with temperature until the temperature at which the enzyme begins to denature is reached, and this is the optimum temperature.

The denaturation temperature depends on the composition of the protein (it's amino acid sequence), which varies for catalase enzymes from different organisms. Most organisms will have some form of catalase, but through different selective pressures and random mutations over millions of years, they all vary slightly. So the answer to your question is that the optimum temperature depends on the source organism.

For most land mammals (cow, pig, human etc) the optimum temperature for any enzyme is likely to be between 36oC and 39oC (most often 37oC). Mammalian catalse has an optimum temperature of about 37oC.

Some examples of other catalase optimum temperatures are as follows (source: www.brenda.uni-koeln.de):

Optimum temperature (oC)

Organism

90

Pyrobaculum calidifontis

90

Thermus brockianus

60

Bacillus sp.

54

Rhodobacter sphaeroides

50

Halobacterium halobium

40

Halobacterium halobium

40

Mycobacterium sp.

40

Vibrio rumoiensis

37

Burkholderia pseudomallei

37

Xanthomonas campestris

30

Bacillus sp.

30

Beta vulgaris var. cicla

30

Mycobacterium sp.

30

Penicillium piceum

30

Synechocystis sp. PCC 6803

30

Synechoystis sp.

25

Helicobacter pylori

25

Trigonopsis variabilis

23

Mycobacterium tuberculosis

22

Escherichia coli

15

Penicillium cyclopium

Answer 2

Many proteins are denatured by temperatures above 40-50°C (but some are still active at 70-80°C, and a few even withstand boiling.

Catalase usually denatures above 38 degrees Celsius.

Answer 3

Catalase is an enzyme that catalyzes the breakdown of hydrogen peroxide. It has an optimum temperature of 37 degrees Celsius, but can increase its activity up to 40 degrees Celsius.

Answer 4

Like almost all enzymes, catalase will denature when subjugated to high temperatures or very acidic environments.

Answer 5

38 degrees celsius

Answer 6

over 38°c

Answer 7

37