The part of the bacterial cell that appears to play the most important role in determining whether an organism if gram-positive or not is the cell wall. it is determined by whether or not it retains the stain during testing.
Cell walls. The peptidoglycan of gram positive bacteria is much thicker, thus holding the Gram stain through the wash. Gram negative bacteria have two thinner walls wuth two periplasam spaces and the stain washes off these bacteria almost completely.
ok decent answer..very simple should i say.
Gram (-)
- thing PG layer
- Single peptide cross link
- outer membrane
- porins
- periplasm (periplasmic space)
-LPS
Gram (+)
-Thick PG layer
- Pentapeptide cross link
- wall and Lipo teichoic acids
biggest differences that should get you through microbiology
Gram stain binds to peptidoglycan, so bacteria with double membranes with thin peptidoglycan layers are more likely not to bind to it, so are usually gram negative, while single membraned bacteria with thick peptidoglycan layers are more likely to be gram positive.
The structure of bacteria that is determined by gram staining is single membrane bacteria that has a thick peptidoglycan surrounding it. When a gram stain is done on a acid-fast bacteria, it normally appears as a gram-positive bacteria.
In Gram+ bacteria, the dark purple crystal violet stain is retained by the thick layer of peptidoglycan which forms the outer layer of the cell.
In Gram- bacteria, the thin peptidoglycan layer in the periplasm does not retain the dark stain, and the pink safranin counterstain stains the peptidoglycan layer.
The cell wall gram positive bacteria contais peptoglycan which reacts with Gram's Stain and develop Purple color which is not washed by alcohol.
Pradeep Kumar
Peptidoglycan
Cell Wall
Gram positive bacteria stain purple due to the chemistry and structure of their petidoglycan cell wall
Gram negative diplocci is the name given to describe the structure and shape of the bacteria, Neisseria gonorrhoeae (the infective organism of the STD gonorrhea) as it is seen on the microscope using a technique call gram staining.
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining
Gram staining is primarily used to differential bacteria based on their cell wall structure. Bacteria are usually classified as Gram positive or Gram negative. Gram positive - thick layer of peptidoglycan as outermost layer, plasma membrane as innermost layer. Gram negative - thin layer of peptidoglycan "sandwiched" in between 2 separate plasma membranes.
Safranin (red) is used in gram staining and endospore staining as the secondary stain. Nigrosin is used in negative staining, staining only the background and not the bacteria. Therefore, the bacteria within the capsule would stain red from the safranin. (Like in endospore staining and negative gram staining, safranin would stain the bacteria red.) Nigrosin would stain the background of the organism just as it would in negative staining. Bacteria (within capsul): stained safranin red Capsule (outer layer of bacteria): clear Background of organism: stained dark with Nigrosin
Gram staining is highly valuable. It allows us to identify two widely different bacteria. Gram staining can tell you if the bacteria is pathogenic or if a penicillin pill can cure it. It tells us gram-positive bacteria, or gram-negative. Positive being easily combated bacteria and some even helpful, and gram-negative being primarily pathogenic.
Gram positive bacteria stain purple due to the chemistry and structure of their petidoglycan cell wall
no, it's eukaryotic. Gram staining is for bacteria.
This is known as a gram test.
gram staining is a biochemical method of identifying bacteria in a more specific way.Thus it is important to differentiate gram positive and gram negative bacteria.
yes it is a special staining like other types....its importance is that one can identify the type of bacteria........
Bacteria are those that are stained dark blue or violet by Gram Staining.
alcohol is organic solvent , it dissolves lipid in cell wall of Gram negative bacteria . this allows out flow of C.V. and counter staining by safranine which results in red color of Gram negative bacteria .
Gram staining is highly valuable. It allows us to identify two widely different bacteria. Gram staining can tell you if the bacteria is pathogenic or if a penicillin pill can cure it. It tells us gram-positive bacteria, or gram-negative. Positive being easily combated bacteria and some even helpful, and gram-negative being primarily pathogenic.
Gram negative bacteria is shaped like rods and stained a reddish pink color due to the gram staining process. Gram negative bacteria do not form spores.
Gram negative diplocci is the name given to describe the structure and shape of the bacteria, Neisseria gonorrhoeae (the infective organism of the STD gonorrhea) as it is seen on the microscope using a technique call gram staining.
Differential staining is the procedure that are used to distinguish organism based on their staining properties. Use of gram stain divide bacteria into two classes - gram positive which retain crystal violet stain purple colour, gram negative which lose their crystal violet and give pink colour. By this method we can differentiate two different types of bacteria having different cell wall composition that is the reason gram staining used widely as differential staining