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When lipids are added to an agar-solidified culture medium and are cultured with lipolytic bacteria, the surrounding medium becomes acidic due to the release of fatty acids. By adding a pH indicator (such as Spirit Blue which has a lavender color), it is possible to detect lipolysis by a clear area surrounding the bacterial growth. A clear area around bacterial growth indicates a positive test. In the absense of lipolysis, the medium retains its lavender color and is a negative reaction.
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If you use glucose broth medium without a pH indicator how can you determine whether a particular bacterium fermented glucose?
you can use a durham tube and observe the formation of a bubble for the microbes that are expected to produce gas
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a bacterium that lives underwater
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bacteria is plural and bacterium is singular
If iodine were not available how would you determine whether starch hydrolysis had occurred?
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How can you determine whether the zone of inhibition is due to death of a bacterium or to inhibition of growth?
How can you determine whether the zone of inhibition is due to death or the effect of the antibiotics?
What 2 parts of a bacterium determine its shape?
cell wall and DNA
If glucose broth medium is used without a pH indicator how can you determine whether a particular bacterium fermented glucose?
you can use a durham tube and observe the formation of a bubble for the microbes that are expected to produce gas
If you use glucose broth medium without a pH indicator how can you determine whether a particular bacterium fermented glucose?
you can use a durham tube and observe the formation of a bubble for the microbes that are expected to produce gas
What foods are spoiled by lipolytic bacteria?
EVERYTHING!
What has the author Arend Jan Slotboom written?
Arend Jan Slotboom has written: 'Application of lipolytic enzymes in the synthesis of monoacyl phosphoglycerides and plasmalogens' -- subject(s): Lipolytic enzymes, Phospholipids
How do you determine whether a rock is a rhyolite or an andesite?
The composition of the rock will determine whether it is a rhyolite or an andesite.
Food that might be spoiled by lipolytic bacteria?
Any food that has a high fat content....
What two parts of a bacterium determine its shape?
cell wall and DNA because bacteria don't have a cytoskeleton
Is a host a living thing as in cells?
Yes, the host is living, whether it be a host for a virus, a worm, or a bacterium.
What is diffusion susceptibility test?
It is also called the Kirby-Bauer test. It is used to determine the effectiveness of an antibiotic against a bacterium.
