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The effect on the bacteria depends if the stain is an acidic or basic stain. Most bacteria are stained when a basic stain permeates the cell wall and adheres by weak ionic bonds to the bacterial cell, which is slightly negatively charged.
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∙ 12y ago
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∙ 14y agoaround neutral pH the bacteria carry an overall negative charge in its surface. since the acid dyes have a chromophore ( color giving organic part of the stain) with a negative charge, it will not stain the bacteria.
Wiki User
∙ 9y agoThe pH will determine if the bacteria will have a particular charge. A negative stain is a simple stain that stains the background but leaves the bacteria unstained.
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∙ 11y agoAcidic stains have a negatively charged chromogen and are repelled by the negative charges on bacterial surfaces.
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∙ 12y agoNo because archaea lack peptidoglycan in their cell walls, which is what the gram stain method is based off of.
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Why an acidic stain is used in negative staining?
Acidic dyes are negatively-charged dyes. Since bacteria are also negatively-charged, they will repel the acidic dyes. So, instead of staining the bacterium itself, it will be the background that will be colorized....
What causes a stain to adhere to bacteria cells and why all colored dyes not necessarily useful for simple staining?
The causes a stain adheres to bacterial cells is the color-bearing ions (chromophores) and they might be positively charged (cationic), such as methylene blue, basic fuchsin, and crystal violet, because bacteria are negatively charged and anionic chromophores, such as eosin, will not stain bacteria because of the electrostatic repelling forces that are involved.
Acidic Congo red does not contain carbon particles like Nigrosin but can give the appearance of a negative stain What is the basis for this stain?
Acidic Congo red is negatively charged, so it is repelled by the negatively charged cytoplasm, and gathers around the cell wall instead, leaving the cell clear and unstained.
Why mitochondria is acidophilic?
Mitochondria is acidophilic because it has membrane proteins which have a net positive charge . Any structures are acidophilic if they stain with eosin, which is a negatively charged dye that stains pink to red.
Can Methylene blue used to stain bacteria?
Yes because Methylene Blue is a symple stain which allows the staining of Cocci. The only thing that is done with the stain is to show the morphology of the bacteria, so one could tell the shape, size, and, arrangement.
Why do bacteria tend to stain better with cationic dyes?
Cationic dyes are positively charged, bacteria must have negatively charged dyes on their surface to attract.
Why an acidic stain is used in negative staining?
Acidic dyes are negatively-charged dyes. Since bacteria are also negatively-charged, they will repel the acidic dyes. So, instead of staining the bacterium itself, it will be the background that will be colorized....
Why microbiologists use stains on cells?
Basic dyes have positively charged chromophore; thus, there target of binding affinity is the specimen or the microorganism which has a negatively charged cell wall. Through this, basic dyes can penetrate and making it an efficient stain for microorganisms.
What causes a stain to adhere to bacteria cells and why all colored dyes not necessarily useful for simple staining?
The causes a stain adheres to bacterial cells is the color-bearing ions (chromophores) and they might be positively charged (cationic), such as methylene blue, basic fuchsin, and crystal violet, because bacteria are negatively charged and anionic chromophores, such as eosin, will not stain bacteria because of the electrostatic repelling forces that are involved.
Acidic Congo red does not contain carbon particles like Nigrosin but can give the appearance of a negative stain What is the basis for this stain?
Acidic Congo red is negatively charged, so it is repelled by the negatively charged cytoplasm, and gathers around the cell wall instead, leaving the cell clear and unstained.
What is the difference between acidic dye and basic dye?
basic dyes are more effective for bacterial staining than acidic dyes because basic dyes have a positive charged chromogen. Bacterial nucleic acids and certain cell wall components carry a negative charge that strongly binds to the cationic chromogen.
Why cant methylene blue be used in place of nigrosin in negative staining?
Because negative staining requires the use of an acidic stain, which will not penetrate the cells because of the negative charge on the surface of the bacteria. As a result, the unstained cells can be easily identified against the colored background.
Why does nigrosin not stain bacterial cells?
Because Nigrosin is an acidic dye--carries a negative charge--and repels against the negatively charged cell walls of most bacteria.
Why is a negative charge dye used to stain bacteria?
because the outer membrane is phospholipid bi layer, which is positive charged one.
Why is mitochondria?
Mitochondria is acidophilic because it has membrane proteins which have a net positive charge . Any structures are acidophilic if they stain with eosin, which is a negatively charged dye that stains pink to red.
Why mitochondria is acidophilic?
Mitochondria is acidophilic because it has membrane proteins which have a net positive charge . Any structures are acidophilic if they stain with eosin, which is a negatively charged dye that stains pink to red.
Can Methylene blue used to stain bacteria?
Yes because Methylene Blue is a symple stain which allows the staining of Cocci. The only thing that is done with the stain is to show the morphology of the bacteria, so one could tell the shape, size, and, arrangement.
